Table 1.

Prespotting settings (to remove excess liquid on the pins)

Parameter	Description	Recommended value
Number of prespots	Spotting times of a freshly loaded pin	6
Delay before spotting	Time of suspended pins above slide	0.5 s
Target height	Distance of pin into contact with slide	15 mm
Speed	Speed of pin into contact with slide	4 mm/s
Surface height	Thickness of slide	1 mm
Dwell time	Time tool is held in place at its target height	0.250 s
Multiple strikes	Number of tapping for each spot	1
Pitch	Distance between the centers of adjacent spots	0.45 mm