Figure 6.

Loss of PYR1 function confers enhanced mitogen-activated kinase activation and PR-1 protein accumulation following P. syringae DC3000 infection. Western blot with anti-pTEpY and anti-PR-1 antibodies of crude protein extracts derived from Col-0, pyr1-2 plants at 0, 24, and 48 h.p.i with P. syringae DC3000. Equal protein loading was check by Ponceau-S staining of the nitrocellulose filter. MPK6 and MPK3 migrating bands are indicated on the right. The experiments were repeated three times with similar results. Scan quantification of protein bands corresponding to MPK3 and PR-1 is shown below the Western blot. Data represent the mean ± SD; n = 3 replicates. An ANOVA was conducted to assess significant differences in RT-qPCR analysis, with a priori p < 0.05 level of significance; the asterisks * above the bars indicate statistically significant differences regarding Col-0 plants.