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. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: Immunity. 2020 Aug 18;53(2):398–416.e8. doi: 10.1016/j.immuni.2020.07.010

Figure 6. Lyz1-deficient microbiota transplanted to Lyz1-intact host, promotes inflammation in experimental colitis.

Figure 6.

(A) Experimental design schematics. (B) PCoA showed the maintenance of diverse communities in GF mice with FMT from the 3 genotypes. (C) Averaged relative abundance of phyla in the Lyz1−/−-FMT inoculum and in the colonized WT GF mice 7 days later. (D) Relative abundance of D. formicigenerans and R. gnavus in fecal microbiota of Lyz1−/−-FMT mice, 7 and day 14 post-FMT as compared to inoculum. (E) In PERMANOVA and ANOSIM analysis, the source/genotype of the inoculum, but not the duration of colonization, determined the microbial differences. (F) Experimental design of DSS colitis in ex-GF mice after FMT with different microbiota (N=5 ex-GF mice-FMT donor genotype). (G-H) Representative H&E images of distal colons and colitis activity scores in DSS-treated ex-GF mice. (I) Body weight changes of ex-GF mice before and after DSS treatment. (J) NanoString analysis of inflammation-related gene expression in the proximal colons of ex-GF mice before and after DSS treatment. (K-L) Areg immunohistochemistry in the colons of DSS-treated ex-GF mice. (M-P) Representative pStat3 and pStat6 immunohistochemistry and semi-quantitative analysis in the colons of DSS-treated ex-GF mice (n=5 in each group). Data are represented as mean ± SEM in D, H, K, O and P from at least two independent experiments. See also Figure S6.