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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Methods Mol Biol. 2012;879:47–69. doi: 10.1007/978-1-61779-815-3_4

Fig. 4.

Fig. 4.

PMML labeling system to generate ISCs clones in the midgut. (a) A schematic diagram showing how to generate a functional actin5C-gal4 gene by using the FLP-mediated FRT recombination technique. A functional actin5C-gal4 gene is reconstituted by heat shock-induced FLP-mediated recombination between inactive but complimentary alleles, actin5C FRT and FRT gal4. The daughter cell that inherits the actin5C-gal4 gene expresses UAS-GFP or any other Iransgene constructs. (b) Gut with GFP-marked wild-type PMML clones. Anti-GFP (green), anti-Arm (red-diploid cell nest), anti-Pros (red-nuclei of ee cells), and Dapi (blue). (c) Gut with GFP-marked UAS-NCA PMML clones. GFP (green), Arm (red-diploid cell nest), anti-Pros (red-nuclei of ee cells), and Dapi (blue). (d) Gut with GFP-marked UAS-NDN PMML clones. Anti-GFP (green), anti-Arm (red-diploid cell nest), anti-Pros (red-nuclei of ee cells), and Dapi (blue). (a) The figure is adapted with permission from Kirilly et al. (58). Scale bars: 10 μm.