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. 2020 Aug 15;21(16):5870. doi: 10.3390/ijms21165870

Table 3.

List of assessed genes and primer sequences evaluated by RT-qPCR in colon samples from TNBS-induced ulcerative colitis protocol.

Gene Primer Sequences
MUC-2

ZO-1
Fw: GATAGGTGGCAGACAGGAGA
Rv: GCTGACGAGTGGTTGGTGATTG
Fw: GGGGCCTACACTGATCAAGA
Rv: TGGAGATGAGGCTTCTGCTT
COX-2 Fw: CGCTTCTCCCTGAAACCTTAC
Rv: GTAGAGGGCTTTCAACTCTGCA
IFN-γ

iNOS
Fw: ATGAGTGCTACACGCCGCGTCTTGG
Rv: GAGTTCATTGACAGCTTTGTGCTGG
Fw: GCTACACTTCCAACGCAACA
Rv: GTGGGAGGGGTAGTGAT
SOCS3 Fw: CCTCCAGCATCTTTGTCGGAAGAC
Rv: TACTGGTCCAGGAACTCCCGAATG
NFκB (p65) Fw: CTGGCAGCTCTTCTCAAAGC
Rv: CCAGGTCATAGAGAGGCTCAA
GAPDH Fw: CCATCACCATCTTCCAGGAG
Rv: CCTGCTTCACCACCTTCTTG