Fig 4.

HMGB1 is a direct target of miR-142-3p. (A) Diagram of the binding site between miR-103a-3p and HMGB1 3’-UTR. (B) Dual-luciferase reporter assay of miR-103a-3p and HMGB1 3’-UTR. 293T cells were co-transfected with miR-103a-3p mimics and WT or MT reporter vector. Luciferase activity was measured after 48 h incubation. (C) Quantitative real-time PCR and (D) Western blot analysis of HMGB1 expression in chondrocytes transfected with miR-103a-3p mimics in the presence with LPS. **p < 0.01, ***p < 0.001, as compared with control; #p < 0.05, as compared with LPS+miR-NC (E) Relative HMGB1 protein level.