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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Mol Endocrinol. 2020 Oct;65(3):59–67. doi: 10.1530/JME-20-0082

Fig 5. G3R1 knockout mice lack a detectable islet phenotype.

Fig 5.

(A) CRISPR strategy for deletion of G3R1 exon 2, and genotyping indicating successful creation of a mouse lines with >80% deletion of G3R1. WT = Wild type genotyping, KO = Knockout genotyping. (B) Intraperitoneal glucose tolerance test of G3R1-KO mice, and both wild type and heterozygous littermates at 8-weeks of age. +/+ = 8, +/− = 4, −/− = 4. (C) Random blood glucose levels measured at 8-weeks of age. +/+ = 5, +/− = 5, −/− = 7. Error bars indicate standard error of the mean. Immunofluorescence staining of 8-week old pancreas from WT and G3R1-KO mice for (D) INSULIN and GLUCAGON, (E) INSULIN and MAFA, and (F) SYNAPTOPHYSIN and PDX1, as well as DAPI. Images shown are representative.