Table 1.
List of mouse models with Pten mutations included in this review
| Type | Pten allele | Pten perturbation | Site of Pten perturbation | Common name | References | MGI link |
|---|---|---|---|---|---|---|
| Germline | Pten+/− [Ptentm1Rps] | Loss-of-function (haploinsufficiency) | Ubiquitous | Pten+/− | Page et al. 2009; Clipperton-Allen and Page 2014, 2015; Séjourné et al. 2015; Huang et al. 2016 | www.informatics.jax.org/allele/MGI:2151804 |
| Germline | Ptenm3m4 [Ptentm1Engc] | ↓ Nuclear: cytoplasm ratio | Ubiquitous | Ptenm3m4 | Mester et al. 2011; Tilot et al. 2014 | www.informatics.jax.org/allele/reference/J:210487 |
| Germline | Ptenαmu | Deletion of PTENα isoform | Ubiquitous | Ptenαmu | Wang et al. 2017 | n/a |
| Germline | PtenTg [Tg(Pten)1Srn] | Overexpression | Ubiquitous | PtenTg | Sanchez-Puelles et al. 2019 | www.informatics.jax.org/allele/MGI:5645732 |
| Germline | Pten ΔPDZ [Ptentm1.1Jaes] | Deletion of PDZ-binding domain | Ubiquitous | PtenΔPDZ | Sanchez-Puelles et al. 2019 | www.informatics.jax.org/allele/key/868490 |
| Conditional (PtenloxP) | Ptentm2Mak | Loss-of-function | CB and DG granule cells, some CTX neurons [Tg(Gfap-cre)1Sbk] | NS-Cre; PtenloxP | Backman et al. 2001; Kwon et al. 2001, 2003; Ljungberg et al. 2009; Lugo et al. 2014, 2017; Nguyen et al. 2015; Smith et al. 2016; Binder and Lugo 2017; Hodges et al. 2018 |
Tg(Gfap-cre)1Sbk: www.informatics.jax.org/allele/MGI:2448664 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 |
| Conditional (PtenloxP) |
Ptentm2Mak Ptentm1Hwu |
Loss-of-function | Mature CTX and HIPP neurons [Tg(Eno2-cre)39Jme, Tg(Eno2-cre)2Lfp] | Nse-Cre; PtenloxP | Kwon et al. 2006; Ogawa et al. 2007; Zhou et al. 2009; Napoli et al. 2012; Nolan et al. 2019 |
Tg(Eno2-cre)39Jme: www.informatics.jax.org/allele/MGI:2177175 Tg(Eno2-cre)2Lfp: www.informatics.jax.org/allele/MGI:3621475 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm2Mak | Loss-of-function | Inducible; postnatal NSCs in SGZ, SVZ [Tg(Nes-cre/ERT2)73Lfp] | Nestin-Cre; PtenloxP | Amiri et al. 2012 |
Tg(Nes-cre/ERT2)73Lfp: www.informatics.jax.org/allele/MGI:3840184 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | NSCs [Tg(Gfap-cre)77.6Mvs] | NSC-Cre; PtenloxP | Gregorian et al. 2009 |
Tg(Gfap-cre)77.6Mvs: www.informatics.jax.org/allele/MGI:3838840 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm1.1Mwst | Loss-of-function | Mesenchymal cells and WM astrocytes [Tg(S100a4-cre)1Gle] | Fsp1-Cre; PtenloxP | Borniger et al. 2016 |
Tg(S100a4-cre)1Gle: www.informatics.jax.org/allele/MGI:3775823 Ptentm1.1Mwst: www.informatics.jax.org/allele/MGI:4366755 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | NeoCTX and HIPP neurons, subset of glia [Emx1tm1(cre)Krj] | Emx1-Cre; PtenloxP | Huang et al. 2016 |
Emx1tm1(cre)Krj: www.informatics.jax.org/allele/MGI:2684610 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | Oxytocinergic neurons [Oxttm1.1(cre)Dolsn] | Oxt-Cre; PtenloxP | Clipperton-Allen et al. 2016 |
Oxttm1.1(cre)Dolsn: www.informatics.jax.org/allele/MGI:5523143 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | Purkinje cells [Tg(Pcp2-cre)2Mpin] | L7-Cre; PtenloxP | Cupolillo et al. 2016 |
Tg(Pcp2-cre)2Mpin: www.informatics.jax.org/allele/MGI:2137515 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | Dopaminergic neurons [Slc6a3tm1.1(cre)Bkmn] | DAT-Cre; PtenloxP | Diaz-Ruiz et al. 2009; Clipperton-Allen and Page 2014 |
Slc6a3tm1.1(cre)Bkmn: www.informatics.jax.org/allele/MGI:3689434 Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| Conditional (PtenloxP) | Ptentm2Mak | Loss-of-function | Subsets of DG, HAN, AMYG neurons [Tg(Pomc-cre)1Lowl] | Pomc-Cre; PtenloxP | Matsushita et al. 2016 |
Tg(Pomc-cre)1Lowl: www.informatics.jax.org/allele/MGI:4362028 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 |
| Conditional (PtenloxP) | Ptentm2Mak | Loss-of-function | Excitatory neurons [Tg(Camk2a-cre)T29-1Stl] | CaMKIIa-Cre; PtenloxP | Sperow et al. 2012 |
Tg(Camk2a-cre)T29-1Stl: www.informatics.jax.org/allele/MGI:2177650 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 |
| Conditional (PtenloxP) | Ptentm2Mak | Loss-of-function | MGE and MPOA progenitors [Tg(Nkx2-1-cre)2Sand] | Nkx2.1-Cre; PtenloxP | Vogt et al. 2015 |
Tg(Nkx2-1-cre)2Sand: www.informatics.jax.org/allele/MGI:3773076 Ptentm2Mak: www.informatics.jax.org/allele/MGI:2182005 |
| Conditional (PtenloxP) | Ptentm1Hwu | Loss-of-function | AAV-Cre in somatosensory CTX at P1 | n/a | Gutilla et al. 2016 | Ptentm1Hwu: www.informatics.jax.org/allele/MGI:2156086 |
| shRNA targeting Pten | n/a | shRNA knockdown | BLA, LA | n/a | Haws et al. 2014 | n/a |
(CB) cerebellum, (DG) dentate gyrus, (CTX) cortex, (HIPP) hippocampus, (NSC) neural stem cells, (SGZ) subgranular zone, (SVZ) subventricular zone, (WM) white matter, (HAN) hypothalamic arcuate nucleus, (AMYG) amygdala, (MGE) medial ganglionic eminence, (MPOA) medial preoptic area, (shRNA) short hairpin RNA, (BLA) basolateral amygdala, (LA) lateral amygdala.