FIGURE 7.

Decidualized cells condition monocyte-derived cells to an immunosuppressive and tolerogenic profile with induction of CD4⁺HLA-G⁺ T cells after allogeneic stimulation. Monocyte-derived cells (5 × 10⁴ cells/100 μl) that had been differentiated in presence or absence of 1:2 dilution of Non-Dec or Dec-CM for 6 days were suspended in DC complete medium and co-cultured with allogeneic lymphocytes for 5 days more (DC/lymphocyte ratio = 1/5). On the last day of MLR, the expression of CD4, CD25, and HLA-G was evaluated by flow cytometry, gating on lymphocytes. Representative dot plots are shown in panels (A,C). Bars represent the mean ± SEM of positive cells from four experiments (B,D). The statistical test used is the Friedman test with Dunn’s multiple comparisons post-test. Exact p-values and comparisons are indicated in the graph.