TABLE 2.

Summary of the relevant articles on the application of next-generation sequencing to the pesticide effect on soil microorganisms.

References	Substance	Type	Study	Comments
Wang et al. (2020)	Azoxystrobin	Fungicide	Lab	Significant dose dependent impact of the substance on the bacterial community diversity and changes in its composition following exposure to specialized degraders.
Farthing et al. (2020)	Glyphosate and imidazolinone	Herbicide	Field	Transient significant and overall little impact following treatments. Changes mostly attributed to the field properties.
Fang et al. (2020)	Chloropicrin, dazomet, dimethyl disulfide, allyl isothiocyanate and 1,3-dichloropropene	Fumigant	Lab	Transient significant changes with initial diversity decline and brief stimulation. Fumigant type dependent various responses from soil bacterial community and its denitrifiers.
Zhang D. L. et al. (2019)	1,3-dichloropropene	Fumigant	Field	Bacterial community composition remained unaffected by 1,3-D fumigation whereas its impact was detrimental to biodiversity of bacteria, AOA-amoA and AOB-amoA genes.
Zhang C. et al. (2019)	Pyraclostrobin	Fungicide	Lab	Significant impact in the abundances of genera with important roles in soil fertility and pollutant biodegradation.
Tang et al. (2019)	Glufosinate (glyphosate)	Herbicide	Lab	Some variations in bacterial diversity of rhizosphere caused only by plant growth stages. These changes were not attributed to treatments.
Mallet et al. (2019)	Leptospermone	Herbicide	Lab	Significant shifts in community structure and diversity in fungal communities of soils caused by natural weed killer leptospermone. Recovery was only possible for the soil in which indigenous fungal community prior to experiments was already diverse and rich.
Hu et al. (2019)	Fomesafen	Herbicide	Field	A dose dependent effect impact on diversity. Long-lasting significant impact on the soil microbial community and changes toward specialized microorganisms that are able to degrade Fomesafen.
Gallego et al. (2019)	Oxamyl	Nematicide	Field	Increased abundance of the specialized fraction and transient changes in the composition total bacterial community.
Feng et al. (2019)	Lindane	Insecticide	Field	Bacteria were than fungi but stable community structure exhibited in the hybrid rice under lindane stress.
Storck et al. (2018)	Chlorpyrifos, isoproturon, and tebuconazole	Insecticide; herbicide; fungicide	Field	Diversity and composition varied over time more in mesocosms than field. Overall, all pesticides referred as low-risk.
Jeffries et al. (2018)	Chlorpyrifos	Insecticide	Field	Legacy effect after 13 years, community that is able to adapt and degrade OP is still reflected.
Zhang S. T. et al. (2017)	Chloropicrin	Fumigant	Field	Richness and diversity after 3 years continuous fumigation were the lowest. Increase of fumigation years reduced the incidence of bacterial wilt.
Jiang et al. (2017)	Acetochlor (2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl) acetomide)	Herbicide	Field	No significant impact on soil microbial community composition after 9 years of treatments. Changes between 8th and 9th year were found to be not related to herbicide but to a seasonality.
Chen et al. (2017)	Paichongding neonicotinoid	Insecticide	Lab	Significant, both positive and negative, soil type dependent impact on soil bacterial community. Diversity was found to be gradually increasing in control group while it was decreasing in the IPP group. The inoculation of an IPP degrading strain positively affected the microbial species diversity in contaminated soil.
Panelli et al. (2017)	Farming systems analysis	Various	Field	Fungal and bacterial community alterations caused by various treatments, exact comparison of two systems were not possible.