Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 18;14:848. doi: 10.3389/fnins.2020.00848

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Wang, Ru, Zhang, Chen, Lin, Lin, Wen, Huang, Ni, Zhuge and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Administration of basic plasma exosomes (EXO1) and melatonin-treated plasma exosomes (EXO2) inhibited NLRP3-dependent pyroptosis in the ischemic cortex. (A) dUTP nick-end labeling (TUNEL) assay was used to detect the apoptosis of cortical nerve cells post-ischemic injury. Representative images of the TUNEL-positive apoptotic cells (red) in sagittal brain sections at day 3 post-injury. The nuclei of all cells were stained with DAPI (blue, n = 5. (B) Comparison of the number of TUNEL-positive cells with EXO1 or EXO2 treatment. (C) Lactate dehydrogenase (LDH) release for the detection of cell membrane pore formation (n = 5). (D–H) Western blot analysis of NLRP3, ASC, active caspase-1, and active GSDMD (N-terminal, n = 5). Data are presented as the mean ± SD. ***P < 0.001 vs. the permanent distal middle cerebral artery occlusion (pMCAO) group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the EXO1 group.