Skip to main content
. 2020 Aug 18;7:199. doi: 10.3389/fmolb.2020.00199

FIGURE 2.

FIGURE 2

GCLC was dramatically decreased in liver tissues of patients with HCV-related liver fibrosis. Liver tissues from healthy liver transplant donor (n = 5) and patients with HCV-related liver fibrosis (n = 8) were detected. (A) Liver fibrosis was evaluated with Masson staining, and expression of α-SMA, COL1, and GCLC were detected by IHC. Positive staining was indicated by brown color. (B–D) Expression of α-SMA, COL1, and GCLC in liver tissues of HCV patients was detected by qRT-PCR (B) and Western blot (C,D). (E–G) Expression of ER stress-related genes in liver tissues of HCV patients was detected by qRT-PCR (E) and Western blot (F,G). (H–J) Expression of inflammatory factor NF-κB, IKKB, and TNFα in liver tissues of HCV patients was detected by qRT-PCR (H) and Western blot (I,J). (K–M) Expression of fibrotic genes such as TGFβ1, MMP2, and TIMP1 in liver tissues of HCV patients was detected by qRT-PCR (K) and Western blot (L,M). *P < 0.05, **P < 0.01. HC: healthy control.