FIGURE 2.

Amplification assays using RNA preparations from FHVd-LR-positive and -negative samples, and primer pairs FHVd-5F/6R (left) or FHVd-3F/4R (right). Lane 1, amplification was performed in the absence of reverse transcription (−RT) using an FHVd-LR-positive sample. Lanes 2 and 3, amplifications were performed after reverse transcription (+RT) using RNA preparations from FHVd-LR-negative or -positive samples, respectively. Lane 4 is a non-template control. The same FHVd-LR-positive sample was tested in lanes 1 and 3.