FIGURE 1.

Transcriptome profiling of CD4⁺T cells in KO and WT mice. (A) T cell-specific AdipoR1 knockout (KO) mice were generated using the CD4-Cre/AdipoR1 flox system and their phenotypes analyzed. (B) Relative AdipoR1 mRNA expression in CD4⁺ cells and CD4^– cells was measured by RT-PCR (n = 5). (C) AdipoR1 protein levels were measured by western blot (n = 3). (D) Heat map analysis of 646 significantly differentially expressed genes. Individual mouse samples are shown in columns and differentially expressed genes in rows. Red indicates increased expression. Green indicates decreased expression (n = 3). (E) Top genes and transcripts in the Th17 pathway. (F) RT-PCR assay to validate RNA-seq results (n = 4–5). All data are shown as mean ± SD (*p < 0.05, **p < 0.01, and ***p < 0.001).