Skip to main content
. 2020 Aug 18;11:1855. doi: 10.3389/fimmu.2020.01855

Figure 1.

Figure 1

Selection of IL-17 epitopes and characterization of IL17.1 and IL17.2 nanofibers. Two peptide epitopes, IL17.1 and IL17.2, were identified within mouse IL17A. (A) Homologous epitopes are shown within the crystal structure of human IL17A. (B–F) Both IL17.1-Q11 and IL17.2-Q11 peptides self-assembled into nanofibers (imaged using negative-stained TEM) alone or when co-assembled with Q11 and PADRE-Q11 (single peptides formulated at 2 mM; co-assembled peptides formulated at 1 mM IL17.1-Q11 or IL17.2-Q11/0.95 mM Q11/0.05 mM PADRE-Q11; all nanofibers diluted 10-fold before application to TEM grids). (G) All formulations had significant β-sheet character by ThT fluorescence (mean ± SD, n = 3, all groups significantly different from all other groups, ***p < 0.001 by one-way ANOVA and Tukey's Multiple Comparisons test).