Figure 4.

MiR-193a-5p is a tumor-suppressor gene via inhibiting colorectal cancer (CRC) cell migration and invasion. (A,B) Cell migration and invasion ability using transwell or Matrigel-coated transwell in HCT-8 and SW480 cells treated with miR-193a-5p mimics (left panel, representative pictures of transwell chambers; right panel, average counts from three times of independent test; *p < 0.05, ***p < 0.001). (C,D) Wound healing assay. HCT-8 and SW480 cells were treated with miR-193a-5p mimics for 48 h, and cells were scratched with yellow tips. Images were taken 0, 24, 36, and 48 h after scratching (left panel, representative pictures; right panel, the relative migration distance counts from three times of independent test; *p < 0.05, **p < 0.01). (E) Wound healing assay. HCT-8 cells were transfected with miR-193a-5p inhibitor for 48 h and then scratched with yellow tips. Images were taken 0, 24, 36, and 48 h after scratching (left panel, representative pictures; right panel, the relative migration distance counts from three times of independent test; *p < 0.05). (F) Epithelial–mesenchymal translation (EMT)-associated genes expression by western blotting, including N-cadherin, Vimentin, and MMP2.