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. 2020 Aug 18;11:1281. doi: 10.3389/fphar.2020.01281

Figure 4.

Figure 4

CQ decreases the role of NLRC5 in autophagy (n = 5). (A, B) Representative western blotting and qRT-PCR results showing treatment with 30μM autophagy inhibitor CQ restricted the level of LC3-II in NLRC5-overexpressed EESCs compared with NLRC5 plasmid group (**P < 0.01 vs. vector group and ##P < 0.01 vs. NLRC5 group). (C, D) Representative western blotting and qRT-PCR results showing treatment with 30μM autophagy inhibitor CQ inhibited the level of LC3-II in NLRC5-down-regulated EESCs compared with siRNA-NLRC5 group (**P < 0.01 vs. scrambled-RNAi group and ##P < 0.01 vs. siRNA-NLRC5 group).The expression levels of mRNA were normalized with respect to β-actin and were calculated using the 2-ΔΔCt method. The protein expression levels were quantified by Image J software and normalized to β-actin protein levels. The results are represented as the mean ± SEM from at least three independent experiments.