FIGURE 3.
BRG1 regulates SPON2 expression in cultured endothelial cells. (A,B) EAhy926 cells were transfected with wild type (WT) or enzyme deficient (ED) BRG1 followed by treatment with oxLDL (50 μg/ml) for 24 h. SPON2 expression was measured by qPCR and Western. (C,D) EAhy926 cells were transfected with wild type (WT) or enzyme deficient (ED) BRG1 followed by treatment with TNF-α (10 ng/ml) for 24 h. SPON2 expression was measured by qPCR and Western. (E,F) EAhy926 cells or HAECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with oxLDL (50 μg/ml) for 24 h. SPON2 expression was measured by qPCR and Western. (G,H) EAhy926 cells or HAECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with TNF-α (10 ng/ml) for 24 h. SPON2 expression was measured by qPCR and Western. *p < 0.05.