FIGURE 6.

Egr-1 mediates SPON2 transcription to regulate macrophage trafficking. (A–C) EAhy926 cells were transfected with siRNA targeting Egr-1 or scrambled siRNA (SCR) followed by treatment with oxLDL (50 μg/ml) for 24 h. SPON2 expression was measured by qPCR and Western. Recombinant SPON2 was added to the supernatant. Macrophage migration assay was performed and quantified as described in Section “Materials and Methods.” (D–F) EAhy926 cells were transfected with siRNA targeting Egr-1 or scrambled siRNA (SCR) followed by treatment with TNF-α (10 ng/ml) for 24 h. SPON2 expression was measured by qPCR and Western. Recombinant SPON2 was added to the supernatant. Macrophage migration assay was performed and quantified as described in Section “Materials and Methods.” *p < 0.05.