Figure 2.

Expression of Id1a, Id1b, PTBP3, and Hes1 in the differentiating gastric cancer cells. (A) Western blot analysis of Id1a, Id1b, PTBP3, and Hes1 expressed in NaBU-treated MKN45 cells. The cells were treated with 3 mM NaBU for 6–72 h. (B) RT-PCR was performed to display the relative levels of Id1a and Id1b mRNAs in NaBU-treated MKN45 cells. (C) The map showed the primer sites for RT-PCR. Id1a and Id1b proteins differ at the C-terminus due to differential splicing of the 239 bp intron (sequence from NCBI).