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. 2020 Aug 18;11:1850. doi: 10.3389/fimmu.2020.01850

Figure 3.

Figure 3

Venus reporter expression in the TEC compartment of immunodeficient RV-SRG mice. Thymic epithelial cells were isolated (108) and analyzed from 2-week old, non-injected RV-SRG mice, or RV-SRG mice that were neonatally injected with proT cells (n = 3–4 mice per group). RANK Venus− mice that were non-injected or proT cell-injected were used for gating controls. (A) Flow cytometric analysis of CD45, CD7, CD5, CD4, and CD8 for detection of thymic engraftment 2-weeks after proT cell adoptive transfer. (B) Flow cytometric analysis of CD45−EPCAM+ pre-gated TECs. CD205 was used to define cTECs or bipotent TEC progenitors, while mTECs were broadly defined as MHC Class II+ UEA-1+ (107). (C) Percentage of CD45−EPCAM+RANK Venus+-gated CD205+ cells or CD205−UEA-1+MHCII+ cells in non-injected or proT-injected mice. Asterisks indicate statistical significance between groups and data are plotted as mean ± standard error of the mean (SEM) (*p < 0.05, two-tailed unpaired student's t-test).