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. 2020 Aug 17;31(15-16):863–880. doi: 10.1089/hum.2020.078

Figure 1.

Figure 1.

LyP-1 receptor (p32) and/or TGFβ-1 expression in breast cancer cell lines and patients. (A) LyP-1 receptor (p32) expression in human and mouse breast cancer cells by immunofluorescence staining. In MDA-MB-231 and 4T1 cells, LyP-1 receptors were localized more on plasma membrane (white arrows) and endoplasmic reticulum-like membrane networks (white triangular arrowheads). In MCF-7 cells, they were more enriched in Golgi-like juxtanuclear compartments (white arrows). Scale bar = 25 μm. (B) LyP-1 receptor expression in breast cancer cell lines by Western blot. Note that mouse LyP-1 receptors in 4T1 cells have a slightly different banding pattern from those in human breast cancer cells. All experiments were repeated three times. (C) Representative images of LyP-1 receptor and TGFβ-1 expression in breast cancer patient samples. Yellow arrows point to positive p32 IHC staining in tumor cells; red open arrows point to TGFβ-1 staining in tumor stroma. Magnification is indicated by scale bars. IHC, immunohistochemistry; TGFβ-1, transforming growth factor β-1.