FIG. 4.

Brain pericytes derived from human pluripotent stem cells. (A) Flow cytometry result showing the positive expression of PDGFRβ in the derived cells. (B) Immunofluorescence images of NG2 expression. Scale bar: 50 μm. (C) A list of expressed genes associated with brain pericytes in cortical organoids with isogenic microglia.¹²¹ The FPKM normalized values for these genes are listed for both samples (MG, microglia-like cell; D-MG, cortical organoids with microglia). The numbers are the Log2 values of ratios of D-MG to MG. Negative values indicate that the genes are present in higher amounts in MG group, while positive values indicate that the genes are present in higher amounts in D-MG group. NG2, neural/glial antigen 2; FPKM, fragments per kilobase per million reads. Color images are available online.