Figure 5.

Neuronal morphology is altered in TUBB4A mutations causing neuronal phenotypes. Cerebellar granule neurons were cultured and transfected wild type and mutant TUBB4A-GFP. Neuronal morphology was visualized by GFP (green channel), and MAP2 immunostaining (red channel) is used to differentiate dendrites vs. axons (white arrows). A scale bar (10 μm) is shown. (G) Quantification of morphology of cerebellar granule neurons is shown including axon length, and dendrite numbers and branch point. Error bars represent standard error of the mean, n = 4, at least 15 transfected neurons were counted for a total of >60 neurons per condition, *P < 0.05, **P < 0.01, calculated by one way ANOVA).