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. 2020 Sep 1;34(17-18):1190–1209. doi: 10.1101/gad.333906.119

Figure 4.

Figure 4.

Phf21b is essential for cell cycle exit during neurogenesis. (A) Scheme indicating the paradigm used. In utero electroporation was conducted at E13.5. Cumulative BrdU (magenta lines) was injected either 3 or 9 h before sacrifice at E15.5 (X). Single injections of EdU (cyan lines) were administered 4 h before sacrifice. (B,C) Representative brain slices electroporated either with shNTC or shPhf21b (green) subjected to cumulative BrdU injections for 9 h, and counterstained for BrdU (magenta) and Tbr2 (red). Empty arrows point to apical progenitors (Tbr2−), which incorporated BrdU. Arrows indicate basal progenitors (Tbr2+) that incorporated BrdU. (D,E) BrdU labeling index for apical progenitors (Tbr2−) or basal progenitors (Tbr2+). (***) P = 0.0007, t-test. Data are represented as mean ± SD from at least three embryos obtained from three different litters. (F) High-power image at the ventricular border of an electroplated brain subjected to EdU injection 4 h before sacrifice. Counterstains show EdU (cyan) and PH3 (red). (G) Apical progenitors EdU mitotic index for the indicated time points using Tbr2 staining. Scale bars: B,C,D, 20 μm.