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. 2020 Aug;30(8):1191–1200. doi: 10.1101/gr.260174.119

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© 2020 Nip et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Assembly quality on mouse simulated bulk RNA-seq data. (A) True positive rate calculated based on I95, denoted as TPR95. (B) True positive rate at four transcript expression strata. Isoforms in strata 1, 2, 3, and 4 have nonzero values of transcripts per million (TPM) in the lowest quartile, second-lowest quartile, second-highest quartile, and the highest quartile, respectively. (C) False-discovery rate calculated based on I95, denoted as FDR95. (D) Misassembly rate calculated based on I95, denoted as MR95.