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. 2020 Aug;30(8):1191–1200. doi: 10.1101/gr.260174.119

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© 2020 Nip et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Assembly sensitivity on experimental data of 96 mouse embryonic stem cells with ERCC and SIRV spiked-in transcripts. (A) Number of spiked-in transcripts reconstructed to at least 50% annotated length, denoted as S50. (B) Number of spiked-in transcripts reconstructed to at least 95% annotated length, denoted as S95. Distributions of each metric were measured over all 96 cells. RB(ref) and RB(ref,pool) assemblies were guided by the mouse transcriptome reference. The comparison bars on top indicate statistical significance of the difference between distributions at P < 0.001 (***), P < 0.01 (**), P < 0.05 (*), or no significance (NS) using the Wilcoxon test.