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4E9 does not cross-react with human CD19. The human B-cell line Nalm6 was labeled with an antihuman CD19 antibody (positive control, left plot, dark gray histogram) or 4E9 (right plot, dark gray histogram). The light gray histogram represents unstained cells, which is used as a negative control. Clear labeling is observed with an antihuman CD19 antibody, but no labeling is seen with 4E9.
