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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Vet Pathol. 2020 Feb 21;57(2):241–252. doi: 10.1177/0300985819900352

Figure 11.

Figure 11.

Single-chain variable fragments (scFvs) derived from 4E9 mAb maintain antigen-binding specificity. Flow cytometry plots of CLBL-1 cells labeled with an irrelevant anti-murine B-cell maturation antigen (BCMA)–specific scFv phage (negative control), a variable light (VL)–variable heavy (VH) 4E9 scFv phage, or a VH-VL 4E9 scFv phage, as indicated. Bound phage was detected with an anti-M13 mAb. Minor background labeling is observed with the negative control BCMA scFv. Both orientations of the 4E9 scFv label CLBL-1 cells, with the VL-VH scFv phage showing greatest binding.