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. 2020 Jul 13;2(1):vdaa087. doi: 10.1093/noajnl/vdaa087

Figure 4.

Figure 4.

Expression levels of genes and proteins of interest in tumor and FACS isolated samples. (A) Expression profiles across tumor regions and in 5-ALA-positive cells for selected genes demonstrating expression patterns varying in the unsorted invasive region and particularly in 5-ALA-positive cells isolated by FACS. (B and C) Immunohistochemistry for VEGFA (×40 images) for a core (B) and invasive area (C) from the same GBM demonstrating representative high and low expression of VEGFA, respectively. (D) RNAseq expression profiles for the PDL1 immune checkpoint molecule, showing high PDL1 expression in the tumor core and low expression in 5-ALA-positive invasive cells (t-test P < .05, indicated by an asterisk). (E and F) Immunohistochemistry for SERPINE1 images (×40 images) showing high SERPINE1 expression in the tumor core (E) and overall low expression in the invasive zone (F); occasional positive cells in the invasive region (arrowhead) are hypothesized to be invasive GBM cells (magnified view of individual positive cell as an insert). (G) Expression levels on RNAseq of SERPINE1 in the unsorted core, rim, and invasive tumor areas and 5-ALA-positive and negative invasive cells, with elevated expression in the 5-ALA-positive FACS isolated population (significance compared to 5-ALA-negative cells t-test P = .014, indicated by an asterisk).