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PDCs retain heterozygous IDH1 R132H through serial passage and continue to produce 2-HG. (A–C) Each of the 3 PDCs retains IDH1 R132H through multiple passages as determined by Sanger sequencing and (D–F) produces 2-HG through multiple passages, as measured by NMR. *The location of the heterozygous G>A mutation in IDH1.
