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. 2020 Aug 20;16(8):e1008810. doi: 10.1371/journal.ppat.1008810

Fig 3. Increased superoxide dismutase (SOD) activity in c14dm‾ parasites.

Fig 3

(A) Whole cell lysates from log phase and stationary phase (day 1-day 3) promastigotes were collected as described in Materials and Methods. SOD activity was measured using a SOD assay kit and results were normalized based on the amount of protein in each sample. Error bars represent standard deviations from two independent experiments. (B) Mitochondria enriched membrane fractions (Mem.) and cytosolic fractions (Sup.) were isolated from log phase promastigotes as described. Western blots were performed using antibodies against L. amazonensis SODA (mitochondrial) and SODB (both SODB1 and SODB2, glycosomal).