Fig 8. C14dm‾ parasites show increased sensitivity to pentamidine (PEN).

(A) Log phase promastigotes were inoculated in M199 media containing various concentrations of PEN. Culture densities were determined after 48 hours and the PEN concentrations required to inhibit growth by 90%, 50%, and 25% were determined (as EC90, EC50 and EC25 respectively) using cells grown in the absence of PEN as controls. “WT + ITZ” represent WT parasites grown in the presence of 0.5 μM of ITZ. (B-C) Log phase promastigotes were inoculated in M199 media containing 2.5 μM (B) or 25 μM (C) of PEN for one hour, followed two washes with PBS. Cells were then re-inoculated in M199 media and culture densities were determined every 24 hours. Some c14dm¯ mutants were also incubated in the presence of 75 μM of CCCp along with PEN (for one hour). Parasites originally inoculated in the absence of PEN were included as controls. Error bars represent standard deviations from three or four independent experiments.