Figure 2.

Effects of alterations in N-glycosylation on the interactions of Chi3l1 or IL-13 with IL-13Rα2. Preconditioned THP-1 cells expressing Chi3l1, IL-13, and IL-13Rα2 were subjected to these evaluations. (A) IP and IB assays on the cells treated with tunicamycin (2 μg/ml, overnight) before harvesting the cells. (B) IP and IB assays on the THP-1 cell lysates treated with Peptide:N-glycosidase F (PNGase F) (1 μl/20 μg of lysates) for 1 hour at 37°C. Each panel is representative of a minimum of three evaluations.