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. 2020 Sep;63(3):374–385. doi: 10.1165/rcmb.2019-0384OC

Figure 4.

Figure 4.

Cell morphology in the competitive repopulation assay. (A–E) Representative images of mature air–liquid interface (ALI) cultures coseeded with UNCCF7T cells and varying percentages of CRC P1 non-CF cells. White, α-tubulin (ciliated cells); green, MUC5AC (mucin 5AC); blue, Hoescht; and red, phalloidin (F actin). Scale bar, 50 μm. (A) 0% non-CF, (B) 1% non-CF, (C) 5% non-CF, (D) 10% non-CF, and (E) 100% non-CF. (F) Orthogonal view of the same representative images. Note the change from a single monolayer, produced by the CF cell line, to a pseudostratified epithelium with more ciliated cells and fewer MUC5AC-expressing cells as more non-CF cells are added. Scale bar, 20 μm. (G) Number of ciliated cells present in the mature ALI culture. (H) Number of MUC5AC+ cells present in the mature ALI culture. Biological n = 3 donors; one replicate per donor. Nine representative fields (291 × 291 μm) per ALI culture were quantified. *P < 0.05 and ***P < 0.001. Data are presented as median and interquartile range.