Table 1.

Statistical Significance of CFTRinh-172 ΔI_sc and Relative Cell Number by ddPCR Analysis

Population	P Values
	1% Non-CF Cells Seeded		5% Non-CF Cells Seeded		10% Non-CF Cells Seeded		100% Non-CF Cells Seeded
	CFTRinh-172 ΔIsc	Relative Cell No.	CFTRinh-172 ΔIsc	Relative Cell No.	CFTRinh-172 ΔIsc	Relative Cell No.	CFTRinh-172 ΔIsc
D1P1 vs. P1	NS	NS	NS	NS	NS	NS	NS
D1P1 vs. P3	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	NS
D1P1 vs. CRC P1	NS	NS	NS	NS	NS	NS	NS
P1 vs. P3	<0.01	<0.01	<0.001	<0.001	<0.001	<0.001	NS
P1 vs. CRC P1	NS	<0.01	NS	<0.01	NS	NS	NS
P3 vs. CRC P3	<0.01	<0.01	<0.001	<0.001	<0.001	<0.001	<0.01
CRC P1 vs. CRC P3	NS	<0.01	NS	<0.001	NS	<0.01	NS

Definition of abbreviations: CF = cystic fibrosis; CFTRinh-172 = cystic fibrosis transmembrane regulator inhibitor-172; CRC = conditionally reprogrammed cell; D1P1 = 1 day to passage 1; ΔI_sc = change in short-circuit current; ddPCR = droplet digital PCR; NS = not significant; P1 = passage 1; P3 = passage 3.

Biological n = 3–4 for the 100% condition; biological n = 6–7 for all other conditions; n = 2–3 for each donor. Two-way ANOVA with Bonferroni’s post hoc test.