Figure 4.

GFP-LC3 expression in neonatal murine lungs. Immunofluorescence staining was performed on cryosections of GFP-LC3 lungs with primary antibodies against SPC (surfactant protein C) (top row), ACTA2 (α-SMA) (second row), FABP4 (fatty acid-binding protein, adipocyte) (third row), p-AMPK (fourth row), and p-S6 (fifth row). The secondary antibody was Alexa Fluor 594 goat antirabbit IgG (red). Representative images are shown. White arrows indicate examples of co-localization of GFP-LC3 (green) with each of the primary antibody targets. Scale bars, 25 μm and 50 μm. A = airway; V = vessel.