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. 2020 Sep;63(3):338–348. doi: 10.1165/rcmb.2019-0445OC

Figure 6.

Figure 6.

Becn1+/− lungs and alveolar macrophages exhibit enhanced inflammatory signaling. (A) BAL fluid (BALF) was obtained from Becn1+/− (KO) and WT littermates after hyperoxia exposure between P1 and P7. Macrophages (Mϕ) and polymorphonuclear neutrophils (PMN) were enumerated on cytospins stained with Diff-Quik. (B) BALF was obtained from other cohorts of hyperoxia-exposed Becn1+/− (KO) and WT littermate mice, and alveolar macrophages were isolated by adhesion purification. Total RNA was isolated, and qRT-PCR was performed for a panel of genes indicated on the figure. (C) Caspase 1 activity was measured in right lung homogenates from hyperoxia-exposed Becn1+/− (KO) and WT littermates using a fluorescence-based activity assay. Relative caspase 1 activity level was expressed as a percentage of activity detected in WT samples. (D) IL-1β protein was measured in whole-lung lysates from hyperoxia-exposed Becn1+/− (KO) and WT littermates using ELISA. All data were derived from a minimum of three different experiments with n = 6–14 per group. Data are shown as mean ± SEM; *P < 0.05 and **P < 0.01.