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. 2020 Sep 1;183(2):315–323.e9. doi: 10.1016/j.cell.2020.08.051

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Figure S4 — Immunological Effects of BCG Vaccination in the Elderly, Related to Figure 3

(A) Healthy volunteers (≥55 years) from the 300BCG cohort were vaccinated with BCG and peripheral blood mononuclear cells (PBMCs) were isolated and stimulated ex vivo with S. aureus or lipopolysaccharide (LPS) of Escherichia coli before vaccination, 14 days (D14) and 90 days (D90) after vaccination. Fold increases (compared to baseline) of interleukin (IL)-1β are shown (n = 14, ^∗p < 0.05 by the Wilcoxon’s ranked sum test). (B) Fold changes of circulating IL-6 and IL-18 upon BCG vaccination as compared to baseline (n = 13). See also Table S3 for a complete list of circulating markers that were measured. (C) Absolute monocyte, granulocyte and lymphocyte counts in whole blood before vaccination, 14 days (D14) and 90 days (D90) after vaccination. Mean values and SE are provided in each panel.