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. 2020 Winter;19(1):18–30. doi: 10.22037/ijpr.2019.14599.12519

Table 3.

The validation parameters and chromatographic properties of MRP and NDM determination methods from plasma samples in literature

Study Analytes LOQ (ng/mL) Accuracy (RE%) Precision (RSD%) Recovery (%) Retention times (min) Linearity (ng/mL) Mobile phase Extraction type Flow (mL/min) Instrument - detector Amount of used plasma (µL) Total analysis time (min)
Ptacek et al. (2003)
[3]
MRP 1.5 (-1.2) – (6.5) MRP, ≤4.9 - - 2 - 151 ACN:30mM PB (2% TEA and SA) pH:4.0 LLE 1 HPLC-FL 1000 4
NDM - - - - -
Romingueres et al. (2002)
[5]
MRP 20 (-4.2) – 2.0 ≤8.3 96.9 4.2 50 - 500 ACN: 6.24 mM PB, pH:6.4 LLE 0.8 HPLC-UV 1000 12
NDM 20 (-9.9) – 1.1 ≤6.1 102.9 2.6 50 - 500
Lavasani et al (2014)
[6]
MRP 1 (-9.98) - 18.3 ≤12.9 86.9 3.5 1 - 500 ACN: 25mM PB, pH:3.0 (20:80, v/v) LLE 2 HPLC-FL 150 5
NDM 2 (-18.9) - 6.8 ≤16.7 37.2 3.0 2 - 500
Frahnert et al. (2003)
[18]
MRP - - ≤7.7 97.9 16.6 5 - 300 ACN: 25mM PB pH:7.0 (40:60, v/v) SPE 1 HPLC-UV 1000 -
NDM - - - - 8.3 -
Titier et al. (2003)
[21]
MRP 25 (-5.5) – (-5.1) ≤11.5 80.5 5.8 25-500 ACN: 10mM PB pH 3.8 LLE 1 HPLC-PDA 1000 18
NDM 25 (-5.5) – (-3.0) ≤12.0 64.0 5.4 25-500
Duvernoil et al. (2003)
[22]
MRP 25 (-0.6) – 5.3 ≤4.74 102.0 6.5 25 - 1000 ACN: PB pH:3.8 LLE 1 HPLC-PDA 1000 18
NDM 20 - - - - -
Pongtanya et al. (2012)
[23]
MRP 3 <15 <15 - - 3 - 300 MeOH:water (0.00002% TFA) LLE 1 HPLC-DAD 1000 -
NDM - - - - - -
Dallet et al. (2002)
[24]
MRP - - - - - - ACN: 25mM PB, pH: 4.8 (10mM TEA) (66:35, v/v) - 1 HPLC-PDA - 20
NDM - - - - - -
Our developed method MRP 0.52 (-0.6) – 5.5 < 3.4 94.4 7.3 10 – 250 MP-A: ACN: 20 mM PB, triethylamine (75:24:1, v/v/v) pH 4.3
MP-B: ACN
LLE 1.2 HPLC-UV 500 12
NDM 0.46 (-2.8) – 3.7 < 2.9 107.0 6.7 10 - 250

ACN: Acetonitrile; PB: Phosphate buffer; LLE: Liquid-liquid extraction; SPE: Solid-phase extraction; HPLC: High performance liquid

chromatography; FL: Flourescent; PDA: Photo diod array; TEA: Triethylamine, TFA: Triflouroacetic acid, SA: Sodium azide, MP: mobile phase.