Figure 6.

Effect of 10058-F4 on ROS level, the cell cycle progression and autophagy system. (A) Upon treatment of NB4 cells with 1008-F4, the intercellular level of ROS was elevated in a concentrations-dependent manner. (B) Treatment of the cells with 10058-F4 increased the proportion of NB4 cells in sub-G1 phase, while there was a reduction in the percentage of cells S phase. (C) The results of BrdU assay showed that 10058‐F4 could hamper the replicative potential of APL cells through reducing DNA synthesis rate. (D) The single agent of 10058-F4 could remarkably diminished the expression level of autophagy-related genes in NB4 cells. Moreover, when autophagy system was suppressed using chloroquine, the cytotoxic effect of 10058-F4 was remarkably reinforced. Values are given as mean ± SD of three independent experiments. ^*P ≤ 0.05 represented significant changes from the control