Figure 4.

Changes in [Ca²⁺]i levels induced by chemical OGD (4 mM NaN₃) in the presence of BD1047 with or without 2 µM noscapine in primary cortical neurons. (A) Time course of NaN₃-induced [Ca²⁺]i rise in presence and absence of 2 µM noscapine and 5 µM BD1047. NaN₃ (4 mM) stimulated [Ca²⁺]i levels increase. After 30 min ischemic insult, the cells treated with 5 µM BD1047. BD1047 could rise [Ca²⁺]i levels during chemical ischemia. Two micromolar noscapine added to 5 µM BD1047 during ischemia and reduced the [Ca²⁺]i levels compare to BD1047 alone. (B) Bar graph of mean change in [Ca²⁺]_i levels acquired in response to chemical ischemia in presence and absence of 2 µM noscapine and 5 µM BD1047. ^***P < 0.001 vs. NaN3 group