Skip to main content
. 2020 Aug 10;16(8):e1008955. doi: 10.1371/journal.pgen.1008955

Fig 2. TM6SF2, ERLIN1 and ERLIN2 function upstream of APOB.

Fig 2

Huh7 cells were transfected with siRNAs targeting TM6SF2 (A), ERLIN1 (B), ERLIN2 (C) or APOB (D) and the indicated plasmids, among which included an siRNA-resistant plasmid that carries a synonymous mutation in the knocked down gene to exclude siRNA off-target effects. After 48 h, cells were fixed, with LDs stained with Bodipy (green) and the transiently expressed proteins with the anti-FLAG antibody (Red). Nuclei were counterstained with DAPI (blue). Scale bars, 10 μm. Cells overexpressing the transfected protein (o/e) and neighboring un-transfected ones (yellow stars) are indicated. Quantification of Bodipy-stained area per cell area is shown on the right. Data are normalized to control cells and presented as mean±SD (n = 2 independent experiments; at least 15 cells per experiment). Student’s t-test. **P<0.01, ***P<0.001, ****P<0.0001, NS, no significance.