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In silico restriction cloning of the gene sequence of final vaccine construct V3 into pET28a(+) expression vector (A: Restriction digestion of the vector pET28a(+) and construct V3 with BglII and BglI, B: Inserted desired fragment (V3 Construct) between BglII (401) and BglI (1943) indicated in red colour. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
