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. 2020 Sep 1;230:113621. doi: 10.1016/j.ijheh.2020.113621

Table 1.

Detection of SARS-CoV-2 RNA by RT-qPCR in untreated wastewater samples from three treatment plants in the metropolitan region of Valencia, Spain.

Sampling date (2020) Water treatment plant Recovery control (%) Ct (N1)a Log10 gc/L ± SD (N1)a Ct (N2)b Log10 gc/L ± SD (N2)b
February 12 Quart-Benàger 2.56 ndc ndc ndc ndc
February 24 Pinedo 1 4.71 ndc ndc ndc ndc
Pinedo 2 7.34 ndc ndc 37.84d 5.22d
March 9 Pinedo 1 5.40 35.16d 5.4d 36.88d 5.45d
Pinedo 2 3.26 34.66,
35.89		 5.37 35.55d 5.77d
March 11 Quart-Benàger 6.31 34.48,
34.80		 5.56 36.95, 36.95 5.43
April 6 Pinedo 1 7.16 34.68,
34.81		 5.53 34.71,
34.69		 5.98
Pinedo 2 4.51 34.81,
34.53		 5.55 36.01,
36.94		 5.55
Quart-Benàger 2.60 35.19,
33.87		 5.59 35.12,
34.17		 5.99
April 9 Pinedo 1 12.07 36.02,
34.73		 5.34 35.55,
36.66		 5.64
Pinedo 2 18.78 34.37,
35.48		 5.47 35.91,
35.51		 5.73
April 11 Quart-Benàger 11.17 34.58,
34.31		 5.62 34.69,
35.52		 5.88
April 13 Quart-Benàger 13.30 33.92,
34.08		 5.75 35.12,
34.66		 5.93
April 14 Pinedo 1 14.52 35.50,
34.79		 5.41 35.82d 5.71d
Pinedo 2 10.54 35.76,
35.2		 5.31 35.12,
36.09		 5.76
a

PCR region encompassing 2019-nCoV_N1 Combined Primer/Probe Mix.

b

PCR region encompassing 2019-nCoV_N2 Combined Primer/Probe Mix.

c

Not detected (Ct > 40).

d

Based on a single technical replicate.