Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Mus. musculus) |
C57BL/6J | Jackson Laboratory | RRID:IMSR_
JAX:000664 |
|
Genetic reagent (Mus. musculus) |
Ctnnb1fl/fl | This paper | Ctnnb1 exons 2–14 floxed. Send reagent request to haihui.xue@hmh-cdi.org | |
Genetic reagent (Mus. musculus) |
Jup1fl/fl | PMID:22036570 | ||
Genetic reagent (Mus. musculus) |
Tcf7fl/fl | PMID:24836425 | ||
Genetic reagent (Mus. musculus) |
Lef1fl/fl | PMID:23103132 | ||
Genetic reagent (Mus. musculus) |
Tcf7l1fl/fl | This paper | Tcf7l1 Exonsix floxed. Send reagent request to haihui.xue@hmh-cdi.org | |
Genetic reagent (Mus. musculus) |
Tcf7l2fl/fl | PMID:21383188 | ||
Strain, strain background (virus) | Lymphocytic choriomeningitis virus Armstrong strain E-350 | ATCC | VR-1271 | |
Antibody | anti-β-catenin (Mouse monoclonal) | BD Biosciences | Cat. No. 610154 Clone 14 |
IB (1:2000) |
Antibody | anti-γ-catenin (Mouse monoclonal) | BD Biosciences | Cat. No. 610253 Clone 15 |
IB (1:2000) |
Antibody | Anti-Tcf4 (Rabbit monoclonal) |
Cell Signaling Technology | Cat. No. 2565 Clone C9B9 |
IB (1:1000) |
Recombinant DNA reagent | MLL-AF9-GFP plasmid | PMID:17463288 | ||
Chemical compound, drug | 4-hydroxy-tamoxifen | Millipore-Sigma | Cat. No. T176 | |
Chemical compound, drug | Tamoxifen | Millipore-Sigma | Cat. No. T5648 | |
Commercial assay or kit | Vybrant FAM caspase-3/7 assay kit | Invitrogen | Cat. No. V35118 | |
Commercial assay or kit | QuantiTect Reverse Transcription Kit | Qiagen | Cat. No. 205313 | |
Software, algorithm | FlowJo | https://www.flowjo.com | RRID:SCR_008520 | |
Software, algorithm | GraphPad Prism | http://www.graphpad.com/ | SCR_015807 |