Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | A549 | Dr. Deepak Nijhawan’s lab at University of Texas Southwestern Medical Center |
Male | |
| Cell line (Homo sapiens) | HCT116 | Dr. Deepak Nijhawan’s lab at University of Texas Southwestern Medical Center |
Male | |
| Cell line (Homo sapiens) | HEK293T | Dr. Deepak Nijhawan’s lab at University of Texas Southwestern Medical Center |
Female | |
| Cell line (Spodoptera frugiperda) | SF9 | Dr. Sanduo Zheng from National Institute of Biological Sciences, Beijing | ||
| Cell line (Trichoplusia ni) | High Five | Dr. Sanduo Zheng from National Institute of Biological Sciences, Beijing | ||
| Cell line (Homo sapiens) | Free style 293 F | Dr. Linfeng Sun at China University of Science and Technology | Female | |
| Antibody | Anti-NRF2 (Rabbit monoclonal) | Abcam (ab62352) | RRID:AB_944418 | WB (1:4000) |
| Antibody | Anti-β-Actin-HRP (Mouse monoclonal) | Huaxingbio (HX18271) | WB (1:10000) | |
| Antibody | Anti-α-Tubulin-HRP (Rabbit polyclonal) | MBL Life Science (PM054-7) | RRID:AB_10695326 | WB (1:10000) |
| Antibody | Anti-DDB1 (Rabbit monoclonal) | Abcam (ab109027) | RRID:AB_10859111 | WB (1:10000) |
| Antibody | Anti-RBX1 (Rabbit polyclonal) | Proteintech(14895–1-AP) | RRID:AB_2179719 | WB (1:5000) |
| Antibody | Anti-UBE2G1 (Rabbit polyclonal) | Proteintech(12012–1-AP) | RRID:AB_10665812 | WB (1:2000) |
| Antibody | Anti-CDK12 (Rabbit polyclonal) | Cell Signaling Technology (11973S) | RRID:AB_2715688 | WB (1:4000) |
| Antibody | Anti-CCNK (Rabbit polyclonal) | Bethyl lab (A301-939A-T) | RRID:AB_2780226 | WB (1:4000) |
| Antibody | Anti-FLAG-HRP (Mouse monoclonal) | Sigma-Aldrich (A8592) | RRID:AB_439702 | WB (1:10000) |
| Antibody | Anti-HA (Mouse monoclonal) | BioLegend (901533) | RRID:AB_2801249 | WB (1:4000) |
| Antibody | Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) (Rat monoclonal) |
Sigma-Aldrich (041571) | RRID:AB_10627998 | WB (1:5000) |
| Antibody | Anti-RNA polymerase II CTD repeat YSPTSPS (Mouse monoclonal) | Abcam (ab817) | RRID:AB_306327 | WB (1:5000) |
| Antibody | Anti-rabbit IgG (Goat polyclonal) | Cell Signaling Technology (7074S) | RRID:AB_2099233 | WB (1:10000) |
| Antibody | Anti-mouse IgG (Goat polyclonal) | Zsbio (ZB-2305) | RRID:AB_2747415 | WB (1:10000) |
| Antibody | Anti-rat IgG (Goat polyclonal) |
Sino Biological (SSA005) | WB (1:10000) | |
| Recombinant DNA reagent | Lenti-EF1α−3xFLAG-CDK12-P2A-BSD WT/GE/GR | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pCDNA3.1-1XFLAG-CDK12 WT/GE/GR |
This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pCDNA3.1-1XFLAG-CDK12-kinase domain (715–1052) WT/GE/GR |
This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pCDNA3.1-3XFLAG-CDK12/13-kinase domain (715–1052/693-1030) WT/GE/GR |
This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pcDNA3.1-CCNK-3xHA | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pET28a-6xHis-Ub | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pCMV-8×His-Ub | Dr. William Kaelin at Dana Farber Cancer Institute of Harvard |
||
| Recombinant DNA reagent | pPB-CAG-1xFLAG-UBA1 | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pPB-CAG-1xFLAG-UBE2D3 | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pPB-CAG-1xFLAG-CUL4A | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pPB-CAG-RBX1 | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pPB-CAG-1xFLAG-Avi-DDB1 | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pFastBac-Strep-8×His-DDB1ΔBPB | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pFastBac-6×His-CDK12KD | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pFastBac-6×His-CCNKΔC | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pFastBac-CAK1 | This paper | Described in Materials and methods; available upon request | |
| Recombinant DNA reagent | pCDNA3.1-CCNK-luc | This paper | Described in Materials and methods; available upon request | |
| Peptide, recombinant protein | HBx peptide | ChinaPetides | ILPKVLHKRTLGLS | |
| Commercial assay or kit | CellTiter-Glo | Promega | G7570 | |
| Commercial assay or kit | AlphaScreen Histidine (Nickel Chelate) Detection Kit | Perkin Elmer | 6760619C | |
| Commercial assay or kit | AlphaScreen Streptavidin Donor beads | Perkin Elmer |
6760619C | |
| Chemical compound, drug | Bortezomib | Targetmol | T2399 | CAS No. 179324-69-7 |
| Chemical compound, drug | THZ531 | Targetmol | T4293 | CAS No. 1702809-17-3 |
| Chemical compound, drug | Dinaciclib | Targetmol | T1912 | CAS No. 779353-01-4 |
| Chemical compound, drug | MLN4924 | Selleckchem | S7109 | CAS No. 905579-51-3 |
| Software, algorithm | BWA | Li, 2013 | v0.7.17 | |
| Software, algorithm | samtools | Li et al., 2009 | v1.10 | |
| Software, algorithm | GATK | DePristo et al., 2011; McKenna et al., 2010; Van der Auwera et al., 2013 | v4.1.7 | |
| Software, algorithm | SnpSift | Cingolani et al., 2012a | v4.3t | |
| Software, algorithm | MAGeCK | Li et al., 2014 | v0.5.9.2 | |
| Software, algorithm | ggplot2 | Wickham, 2016 | ||
| Software, algorithm | GraphPad Prism | v8.0.2 | ||
| Software, algorithm | pLink 2 | Chen et al., 2019 |