Skip to main content
. 2020 Aug 11;9:e57081. doi: 10.7554/eLife.57081

Figure 6. Sfh5 heme center exhibits two magnetic states.

Mössbauer spectra were collected at low-temperature (5 K) and field (0.05 T). (A) Spectrum of Sfh5 sample prepared at pH 7. The red line is a composite simulation with 63% FeIIILA site and 37% FeIIISA site. (B) Spectrum of an independent sample of Sfh5 prepared at pH 8. (C) Spectrum of the sample of Sfh5 in (B) reduced with sodium dithionite. The simulation in B was used to remove 60% of the overall spectral intensity in C. (D) Spectrum of Sfh5H173A100% FeIIILA site. In all spectra, the field was applied parallel to the gamma radiation.

Figure 6.

Figure 6—figure supplement 1. High-field spectrum of 57Fe-enriched Sfh5.

Figure 6—figure supplement 1.

The 4.2 K Mössbauer spectra of Sfh5 at 0 T (A) and 6.0 T (B) applied fields. Red lines are simulations assuming those applied fields at 50% FeIIILA site and 35% FeIIISA site. The simulations did not include 15% of the overall spectral intensity. The field was applied perpendicular to the gamma radiation.