Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Murine anti-Factor IX carboxylated Gla domain (mouse monoclonal) | Green Mountain Antibodies | Cat#GMA-001 | (1:100) |
| Antibody | HRP Anti-beta-actin antibody (mouse monoclonal) | Abcam | Cat#ab20272; RRID:AB_445482 | (1:10,000) |
| Antibody | Amersham ECL Mouse IgG, HRP-linked whole Ab (sheep polyclonal) | GE Healthcare | Cat#NA931; RRID:AB_772210 | (1:10,000) |
| Antibody | Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (goat polyclonal) | ThermoFisher | Cat#:A32723; RRID:AB_2633275 | (1:10,000) |
| Antibody | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (goat polyclonal) | ThermoFisher | Cat#:A-21244; RRID:AB_2535812 | (1:10,000) |
| Antibody | anti-Cofilin (D3F9) XP Rabbit mAb (rabbit monoclonal) | Cell Signaling Technology | Cat#:5175; RRID:AB_10622000 | (1:1000) |
| Antibody | Anti-GFP from mouse IgG1κ (clones 7.1 and 13.1) (mouse monoclonal) | Roche | Cat#:11814460001; RRID:AB_390913 | (1:1000) |
| Antibody | Anti-VKOR (rabbit monoclonal) | PMID:16634640 | (1:1000) | |
| Strain, strain background (Escherichia coli) | E. coli electrocompetent | NEB | Cat#C3020K | |
| Commercial assay or kit | LYNX Rapid APC conjugation kit | BioRad | Cat#LNK033APC | |
| Commercial assay or kit | SuperSignal West Dura Extended Duration Substrate | ThermoFisher | Cat#34076 | |
| Commercial assay or kit | Library Quantification Kit (Illumina) | KAPA Biosystems | Cat#KK4854 | |
| Commercial assay or kit | MiSeq Reagent Kit v3 (600 cycles) | Illumina | Cat#MS-102–3003; RRID:SCR_016379 | |
| Commercial assay or kit | NextSeq 500/550 High Output v2 kit (75 cycles) | Illumina | Cat#TG-160–2005; RRID:SCR_016381 | |
| Commercial assay or kit | Qiagen miniprep kit | Qiagen | Cat#27106 | |
| Commercial assay or kit | GenElute HP Plasmid midiprep kit | Sigma | Cat#NA0200 | |
| Commercial assay or kit | DNA clean and concentrator kit | Zymo | Cat#D4031 | |
| Software, algortihm | PyMOL | Schrodinger | https://pymol.org | |
| Software, algortihm | Enrich2 | PMID:28784151 | https://github.com/FowlerLab/Enrich2 | |
| Software, algortihm | R | R | https://cran.r-project.org/ | |
| Other | Fugene 6 | Promega | Cat#E2691 | |
| Other | Lipofectamine 3000 | ThermoFisher | L3000015 | |
| Other | Atomic coordinates, bacterial VKOR structure | Protein Data Bank | PDB: 4NV5 | |
| Other | Raw and analyzed data | This paper | GSE149922 | |
| Cell line (human) | 293T AAVS1 tetbxb1 clone 4 | PMID:28335006 | ||
| Cell line (human) | HEK293 VKOR activity reporter | PMID:24297869 | ||
| Recombinant DNA reagent | PX458 | Addgene | Cat#48138; RRID:Addgene_48138 |