Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (adeno-associated virus) | AAV1-hsyn-FLEX-CoChR-GFP | UNC vector core | Boyden, E. | |
| Genetic reagent (adeno-associated virus) | AAV9-CAG-FLEX-TdTomato | Penn vector core | ||
| Genetic reagent (adeno-associated virus) | AAV9-Syn-FLEX-jGCaMP7f | Janelia | ||
| Genetic reagent (adeno-associated virus) | AAV9-hSyn-dLight1.2 | Lin Tian | ||
| Chemical compound, drug | Streptavidin cy5 conjugate | Invitrogen | SA1011 | 1:1000 |
| Chemical compound, drug | SR95531 hydrobromide (Gabazine) | Tocris | Cat# 1262 | |
| Chemical compound, drug | SR95531 hydrobromide (Gabazine) | Hello Bio | Cat# HB0901 | used only in Figure 6—figure supplement 1A and B |
| Chemical compound, drug | CGP55845 hydrochloride | Tocris | Cat# 1248 | |
| Chemical compound, drug | D-AP5 | Tocris | Cat# 0106 | |
| Chemical compound, drug | Atropine | MilliporeSigma | Cat# A0132 | |
| Chemical compound, drug | NBQX disodium salt | Tocris | Cat# 1044 | |
| Chemical compound, drug | (±)-Sulpiride | MilliporeSigma | Cat# S8010 | |
| Chemical compound, drug | Tetrodotoxin (TTX) | Tocris | Cat# 1078 | |
| Chemical compound, drug | λ-Aminobutyric Acid (GABA) | MilliporeSigma | Cat# A5835 | |
| Chemical compound, drug | Picrotoxin | MilliporeSigma | Cat# P1675 | |
| Chemical compound, drug | Hexamethonium Chloride | MilliporeSigma | Cat# H2138 | |
| Chemical compound, drug | Diazepam | MilliporeSigma | Cat# D0899 | |
| Chemical compound, drug | Gramicidin | MilliporeSigma | Cat# G5002 | |
| Chemical compound, drug | Muscimol | Tocris | Cat# 0289 | |
| Chemical compound, drug | Gelatin from cold water fish skin | MilliporeSigma | Cat# G7041 | |
| Chemical compound, drug | (+)-Sodium L-ascorbate | MilliporeSigma | Cat# A4034 | |
| Chemical compound, drug | Sodium Pyruvate | MilliporeSigma | Cat# P5280 | |
| Chemical compound, drug | Thiourea | MilliporeSigma | Cat# T7875 | |
| Chemical compound, drug | Triethanolamine | MilliporeSigma | Cat# 90279 | |
| Chemical compound, drug | N,N,N’,N’-Tetrakis-(2-hydroxypropyl)ethylenediamine | TCI | Cat# T0781 | |
| Chemical compound, drug | Urea | MilliporeSigma | Cat# U5128 | |
| Strain, strain background (Mus musculus) | Ai95-RCL-GCaMP6f-D: Cg-Gt(ROSA)26Sor(tm95.1(CAG-GCaMP6f)Hze)/MwarJ | The Jackson Laboratory | Cat# 028865 | male and female over 6 weeks of age |
| Strain, strain background (Mus musculus) | Ai9: Gt(ROSA)26Sor(tm9(CAG-tdTomato)Hze) | The Jackson Laboratory | Cat# 007909 | male and female over 6 weeks of age |
| Strain, strain background (Mus musculus) | TH-GFP (Tg(TH-EGFP)1Gsat | NIH MMRRC | male and female over 6 weeks of age | |
| Strain, strain background (Mus musculus) | C57/Bl6J Wild Type | The Jackson Laboratory | Cat# 000664 | male and female over 6 weeks of age |
| Strain, strain background (Mus musculus) | B6.SJL-Slc6a3(tm1.1(cre)Bkmn/J (DAT-IRES-cre) | The Jackson Laboratory | Cat# 006660 | male and female over 6 weeks of age |
| Strain, strain background (Mus musculus) | Ai32: B6.Cg – Gt(ROSA)26Sor(tm32(CAG-COP4*H143R/EYFP)Hze) | The Jackson Laboratory | Cat#024109 | male and female over 6 weeks of age |
| Software, algorithm | Igor Pro 6 | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | FIJI | PMID:22743772 | RRID:SCR_002285 | http://fiji.sc |
| Software, algorithm | Prism 8 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | pClamp 11 | Axon Instruments | RRID:SCR:011323 |